Summary
Name
Small RNA sequencing of Rice leaves infected with strains of the oryzae or oryzicola pathovars of Xanthomonas oryzae
Description

The sRNA-seq data for the Diversity dataset was generated by the Novogene company (Hong Kong, China) from total RNA extracted from triplicate samples collected at 24 hpi using a NEBNext Small RNA Library Prep Set for Illumina kit optimized by Novogene on the supplied total RNAs. Ultimately, size selected 18–47 bp inserts were sequenced on a Illumina HiSeq (1 × 50 bp).

Program, Pipeline, Workflow or Method Name
Mapping against IRGSP1 genome. sRNA reads coverage--based inferrence of small RNA loci and identification of differentially expressed ones with Deseq2
Program Version
NA
Organism
Author
Ganna Reshetnyak,
Jonathan M. Jacobs
Florence Auguy
Coline Sciallano
Lisa Claude
Clemence Medina
Alvaro L. Perez-Quintero
Aurore Comte
Emilie Thomas
Adam Bogdanove
Ralf Koebnik
Boris Szurek
Anne Dievart
Christophe Brugidou
Severine Lacombe
Sebastien Cunnac
Date Performed
Friday, January 3, 2020 - 15:00
Data Source
Publication
Reshetnyak, G., Jacobs, J.M., Auguy, F. et al. An atypical class of non-coding small RNAs is produced in rice leaves upon bacterial infection. Sci Rep 11, 24141 (2021).