Oryza sativa basmati Basmati334

Basmati 334 (IRGC 27819; GeneSys passport) is a basmati (sensu stricto) landrace from Pakistan and was originally donated to the International Rice Research Institute (IRRI) by the Agricultural Research Council (ARC) in Karachi (donor accession ID: PAK. SR. NO. 39). 

Seeds from accessions IRGC 27819 were obtained from the IRRI seed bank, surface-sterilized with bleach, and germinated in the dark on a wet paper towel for 4 days. Seedlings were transplanted individually in pots containing continuously wet soil in a greenhouse at New York University’s Center for Genomics and Systems Biology and cultivated under a 12-h day-12-h night photoperiod at 30 °C. Plants were kept in the dark in a growth cabinet under the same climatic conditions for 4 days prior to tissue harvesting. Continuous darkness induced chloroplast degradation, which diminishes the amount of chloroplast DNA that would otherwise end up in the DNA extracted from the leaves.

Common Name
Oryza sativa basmati Basmati334
Choi JY, Lye ZN, Groen SC, Dai X, Rughani P, Zaaijer S, Harrington ED, Juul S, Purugganan MD. Nanopore sequencing-based genome assembly and evolutionary genomics of circum-basmati rice.. Genome biology. 2020 02 05; 21(1):21.
Analysis Table
NameProgramDate Constructed
Whole Genome Assembly and Annotation for Basmati334MakerJun 3rd, 2021
InterPro (5.48-83.0) Annotations of Basmati334InterProScanJun 3rd, 2021