Oryza sativa basmati Basmati334

Basmati 334 (IRGC 27819; GeneSys passport) is a basmati (sensu stricto) landrace from Pakistan and was originally donated to the International Rice Research Institute (IRRI) by the Agricultural Research Council (ARC) in Karachi (donor accession ID: PAK. SR. NO. 39). 

Seeds from accessions IRGC 27819 were obtained from the IRRI seed bank, surface-sterilized with bleach, and germinated in the dark on a wet paper towel for 4 days. Seedlings were transplanted individually in pots containing continuously wet soil in a greenhouse at New York University’s Center for Genomics and Systems Biology and cultivated under a 12-h day-12-h night photoperiod at 30 °C. Plants were kept in the dark in a growth cabinet under the same climatic conditions for 4 days prior to tissue harvesting. Continuous darkness induced chloroplast degradation, which diminishes the amount of chloroplast DNA that would otherwise end up in the DNA extracted from the leaves.